1VJC

Structure of pig muscle PGK complexed with MgATP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1999-12-16
Detector	RIGAKU RAXIS II
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	36.200, 106.500, 51.100
Unit cell angles	90.00, 97.60, 90.00

Refinement procedure

Resolution	27.000 - 2.100
R-factor	0.1822
Rwork	0.173
R-free	0.23300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1kf0
RMSD bond length	0.007
RMSD bond angle	1.230
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.000	2.200
High resolution limit [Å]	2.100	2.100
Number of reflections	20849
Completeness [%]	94.1	0.8878

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	287	sodium-citrate, magnesium-chloride, Na-azide, DTT, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 287K