1VJ0
Crystal structure of Alcohol dehydrogenase (TM0436) from Thermotoga maritima at 2.00 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-10-12 |
| Detector | ADSC |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 92.880, 104.919, 161.617 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 80.530 - 2.000 |
| R-factor | 0.14637 |
| Rwork | 0.145 |
| R-free | 0.19390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.591 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (4.2)) |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 92.860 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 106273 | |
| <I/σ(I)> | 11.4 | 3.1 |
| Completeness [%] | 99.2 | 94.3 |
| Redundancy | 3.7 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION,SITTING DROP,NANODROP | 293 | 8.5 Bicine 35 PEG-400 10 Glycerol 5 PEG-3000 , VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 293K |
