1VIW
TENEBRIO MOLITOR ALPHA-AMYLASE-INHIBITOR COMPLEX
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | LURE BEAMLINE DW32 |
| Synchrotron site | LURE |
| Beamline | DW32 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE AREA DETECTOR |
| Collection date | 1997-11 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 168.475, 75.911, 61.989 |
| Unit cell angles | 90.00, 101.29, 90.00 |
Refinement procedure
| Resolution | 10.000 - 3.000 |
| R-factor | 0.23 |
| Rwork | 0.230 |
| R-free | 0.29100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PPA AND ALPHA-AI |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.102 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.843) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 3.100 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.153 | 0.450 |
| Total number of observations | 45403 * | |
| Number of reflections | 14982 | |
| <I/σ(I)> | 4.1 | 1.3 |
| Completeness [%] | 99.3 | 99.3 |
| Redundancy | 3 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 | pH 7 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | sodium cacodylate | 0.1 (M) | |
| 2 | 1 | reservoir | PEG6000 | 13 (%) | |
| 3 | 1 | reservoir | 1 (M) |
