1VIS

Crystal structure of mevalonate kinase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 32-ID
Synchrotron site	APS
Beamline	32-ID
Detector technology	CCD
Detector	MARRESEARCH
Wavelength(s)	0.9794
Spacegroup name	P 1 21 1
Unit cell lengths	56.347, 44.506, 65.880
Unit cell angles	90.00, 103.09, 90.00

Refinement procedure

Resolution	36.570 - 2.690
Rwork	0.275
R-free	0.33700
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.500 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Refinement software	REFMAC (4.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.570	2.530
High resolution limit [Å]	2.400	2.400
Rmerge	0.061	0.034 *
Number of reflections	11993
<I/σ(I)>	8.6	2.1
Completeness [%]	95.2	81.7
Redundancy	2.7	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	HEPES	10 (mM)	pH7.5
2	1	drop		150 (mM)
3	1	drop	methionine	10 (mM)
4	1	drop	glycerol	10 (%)
5	1	drop	dithiothreitol	5 (mM)
6	1	drop	protein	10 (mg/ml)