1VIP

ANTICOAGULANT CLASS II PHOSPHOLIPASE A2 FROM THE VENOM OF VIPERA RUSSELLI RUSSELLI

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU R-AXIS II
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1993-05-08
Detector	RIGAKU
Spacegroup name	I 41 3 2
Unit cell lengths	122.782, 122.782, 122.782
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 - 2.200
R-factor	0.208
Rwork	0.208
R-free	0.23700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1pp2
RMSD bond length	0.010
RMSD bond angle	23.500 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.8)
Refinement software	X-PLOR (3.8)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.240
High resolution limit [Å]	2.200	2.200
Rmerge	0.079 *	0.855 *
Number of reflections	8258
<I/σ(I)>	10.6	3.9
Completeness [%]	99.2	98.5
Redundancy	7.2	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.25		PROTEIN WAS CRYSTALLIZED FROM 1.4 M AMSO4,N50 MM AMOAC AND 2 % DIOXANE AT PH 6.25.

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	1.4 (M)
2	1	reservoir		50 (mM)
3	1	reservoir	dioxane	2 (%)