1VIE
STRUCTURE OF DIHYDROFOLATE REDUCTASE
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU RUH2R |
Temperature [K] | 277 |
Detector technology | AREA DETECTOR |
Collection date | 1991-10-15 |
Detector | XUONG-HAMLIN MULTIWIRE |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 68.740, 68.740, 52.450 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 1.700 |
R-factor | 0.192 |
Rwork | 0.192 |
Structure solution method | MIR |
RMSD bond length | 0.016 |
RMSD bond angle | 16.800 * |
Data reduction software | UCSD |
Data scaling software | UCSD |
Phasing software | X-PLOR |
Refinement software | X-PLOR |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 10.000 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.036 * | |
Number of reflections | 7060 | |
<I/σ(I)> | 13 | 3 |
Completeness [%] | 98.0 | 97 |
Redundancy | 4 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 4 * | CRYSTALS WERE GROWN FROM HANGING- DROPS CONTAINING PROTEIN AT A FINAL CONCENTRATION OF ABOUT 15 MG/ML IN 100 MM TRIS-HCL BUFFER AT PH 7.5 AND 25% 2-METHYL-2,4-PENTANEDIOL (MPD). DROPS WERE EQUILIBRATED AGAINST A RESERVOIR CONTAINING 100 MM KH2PO4 BUFFER AT PH 6.8 AND 50% MPD., vapor diffusion - hanging drop |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 15 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 100 (mM) | |
3 | 1 | drop | MPD | 25 (%) | |
4 | 1 | reservoir | potassium phosphate | 100 (mM) | |
5 | 1 | reservoir | MPD | 50 (%) |