1VHZ

Crystal structure of ADP compounds hydrolase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 32-ID
Synchrotron site	APS
Beamline	32-ID
Detector technology	CCD
Detector	MARRESEARCH
Wavelength(s)	0.9795
Spacegroup name	I 41 3 2
Unit cell lengths	191.773, 191.773, 191.773
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.010 - 2.320
Rwork	0.182
R-free	0.24800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	2.300 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Refinement software	REFMAC (4.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.920	2.440
High resolution limit [Å]	2.320	2.320
Rmerge	0.179	0.338
Number of reflections	26224
<I/σ(I)>	26	13.5
Completeness [%]	99.4 *	96 *
Redundancy	39.9	38.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	HEPES	10 (mM)	pH7.5
2	1	drop		150 (mM)
3	1	drop	methionine	10 (mM)
4	1	drop	glycerol	10 (%)
5	1	drop	dithiothreitol	5 (mM)
6	1	drop	protein	10 (mg/ml)