1VHM

Crystal structure of an hypothetical protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 32-ID
Synchrotron site	APS
Beamline	32-ID
Detector technology	CCD
Detector	MARRESEARCH
Wavelength(s)	0.97942
Spacegroup name	P 43
Unit cell lengths	81.096, 81.096, 51.495
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.620 - 2.100
Rwork	0.206
R-free	0.26800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	2.300 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Refinement software	REFMAC (4.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.620	2.210
High resolution limit [Å]	2.100	2.100
Rmerge	0.065	0.281
Number of reflections	18993
<I/σ(I)>	19	7.4
Completeness [%]	98.0	99.7
Redundancy	7.1	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5 *		100mM MES, 25% PEG 1500, 300mM Sodium Acetate, pH 5.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	HEPES	10 (mM)	pH7.5
2	1	drop		150 (mM)
3	1	drop	methionine	10 (mM)
4	1	drop	glycerol	10 (%)
5	1	drop	dithiothreitol	5 (mM)
6	1	drop	protein	10 (mg/ml)