1VHB
BACTERIAL DIMERIC HEMOGLOBIN FROM VITREOSCILLA STERCORARIA
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | LURE BEAMLINE DW21B |
| Synchrotron site | LURE |
| Beamline | DW21B |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-02 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.320, 41.420, 62.940 |
| Unit cell angles | 90.00, 106.17, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.830 |
| Rwork | 0.184 |
| R-free | 0.25400 * |
| Structure solution method | SIR-DENSITY MODIFICATION |
| RMSD bond length | 0.011 |
| RMSD bond angle | 13.148 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((AGROVATA) |
| Phasing software | MLPHARE |
| Refinement software | TNT (1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.880 |
| High resolution limit [Å] | 1.830 | 1.830 |
| Rmerge | 0.055 | 0.360 |
| Total number of observations | 101071 * | |
| Number of reflections | 26582 | |
| <I/σ(I)> | 12.9 | 2 |
| Completeness [%] | 94.9 | 61 |
| Redundancy | 3.5 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 6.4 | PRECIPITANT: AMMONIUM SULFATE 1.2 M. BUFFER: PYROPHOSPHATE 0.2 M PH 6.4. ADDITIVES: ETHYLENE GLYCOL 3% V/V. PROTEIN CONCENTRATION 25 MG/ML. VAPOR DIFFUSION TECHNIQUES., vapor diffusion |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | ammonium salfate | 1.3 (M) | |
| 2 | 1 | 1 | pyrophosphate | 0.1 (M) | |
| 3 | 1 | 1 | ethylene glycol | 3 (%(v/v)) |
