1VGP
Crystal Structure of an Isozyme of Citrate Synthase from Sulfolbus tokodaii strain7
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL38B1 |
Synchrotron site | SPring-8 |
Beamline | BL38B1 |
Temperature [K] | 90 |
Detector technology | CCD |
Collection date | 2003-12-01 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 121.600, 121.600, 108.830 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.990 - 2.700 |
R-factor | 0.217 |
Rwork | 0.217 |
R-free | 0.24600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1iom |
RMSD bond length | 0.007 |
RMSD bond angle | 1.000 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | CNS |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 54.233 | 2.850 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.097 | 0.470 |
<I/σ(I)> | 1.6 | |
Completeness [%] | 99.3 | 99.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | Formate, HEPES, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |