1VGL
Crystal structure of tetrameric KaiB from T.elongatus BP-1
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARRESEARCH |
Wavelength(s) | 1.0080 |
Spacegroup name | P 1 2 1 |
Unit cell lengths | 63.740, 33.410, 93.740 |
Unit cell angles | 90.00, 100.12, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.600 |
R-factor | 0.23 |
Rwork | 0.227 |
R-free | 0.28900 |
Structure solution method | MAD |
RMSD bond length | 0.013 |
RMSD bond angle | 1.659 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SOLVE |
Refinement software | REFMAC (5.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 2.740 |
High resolution limit [Å] | 2.600 | 2.600 |
Number of reflections | 12369 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 3.8 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | PEG 3350, DMSO, acetate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |