1VGE

TR1.9 FAB FRAGMENT OF A HUMAN IGG1 KAPPA AUTOANTIBODY

Experimental procedure

Detector technology	IMAGE PLATE
Collection date	1995-03-11
Detector	RIGAKU RAXIS II
Spacegroup name	P 1 21 1
Unit cell lengths	50.926, 62.776, 84.790
Unit cell angles	90.00, 107.19, 90.00

Refinement procedure

Resolution	10.000 - 2.000
R-factor	0.18
Rwork	0.180
RMSD bond length	0.016
RMSD bond angle	1.370 *
Data reduction software	XDS
Data scaling software	XDS
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	9999.000 *
High resolution limit [Å]	2.000 *	2.000 *
Rmerge	0.084	0.807 *
Number of reflections	28718	3364 *
Completeness [%]	92.8
Redundancy	3.5	3.5 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	HEPES	10 (mM)
3	1	drop	sodium azide	0.02 (%)
4	1	drop	reservoir solution		0.002 ml
5	1	reservoir	PEG8000	8 (%(w/v))
6	1	reservoir	Tris-HCl	0.1 (M)