1VG7

Crystal Structure Of Octaprenyl Pyrophosphate Synthase From Hyperthermophilic Thermotoga Maritima F132A/L128A/I123A/D62A mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL17B2
Synchrotron site	NSRRC
Beamline	BL17B2
Temperature [K]	100
Detector technology	CCD
Collection date	2004-01-22
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0
Spacegroup name	I 4 2 2
Unit cell lengths	152.935, 152.935, 65.016
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.770 - 3.400
Rwork	0.236
R-free	0.28150
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1v4e
RMSD bond length	0.003
RMSD bond angle	0.840
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	3.520
High resolution limit [Å]	3.400	3.400
Rmerge	0.120	0.480
Number of reflections	5575
<I/σ(I)>	16.78	3.66
Completeness [%]	99.8	99.8
Redundancy	6.47

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	Hepes, Lithium sulfate, PEG 900, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K