1VFY
PHOSPHATIDYLINOSITOL-3-PHOSPHATE BINDING FYVE DOMAIN OF VPS27P PROTEIN FROM SACCHAROMYCES CEREVISIAE
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X9B |
Synchrotron site | NSLS |
Beamline | X9B |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 1999-02-15 |
Detector | ADSC |
Wavelength(s) | 1.2830,1.2822,1.2320 |
Spacegroup name | P 1 |
Unit cell lengths | 24.090, 26.570, 31.610 |
Unit cell angles | 111.79, 92.70, 105.70 |
Refinement procedure
Resolution | 30.000 - 1.150 |
Rwork | 0.174 |
R-free | 0.18100 |
Structure solution method | MAD |
RMSD bond length | 0.012 |
RMSD bond angle | 1.770 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (0.5) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.070 |
High resolution limit [Å] | 1.150 | 2.000 |
Rmerge | 0.065 | 0.011 |
Number of reflections | 20972 | |
<I/σ(I)> | 18.6 | 8 |
Completeness [%] | 95.4 | 82.3 |
Redundancy | 3.8 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.5 * | 4 * | 0.2 M AMMONIUM ACETATE, 0.1 M SODIUM ACETATE PH 4.6, 15% PEG 4000, pH 5.6 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 13-15 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 20 (mM) | |
3 | 1 | drop | dithiothreitol | 10 (mM) | |
4 | 1 | reservoir | ammonium acetate | 0.2 (M) | |
5 | 1 | reservoir | sodium acetate | 0.1 (M) | |
6 | 1 | reservoir | PEG4000 | 15 (%) |