1VFR

THE MAJOR NAD(P)H:FMN OXIDOREDUCTASE FROM VIBRIO FISCHERI

Experimental procedure

Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6B
Synchrotron site	Photon Factory
Beamline	BL-6B
Temperature [K]	285
Detector technology	IMAGE PLATE
Collection date	1995-04
Detector	FUJI
Spacegroup name	C 1 2 1
Unit cell lengths	101.600, 63.300, 74.400
Unit cell angles	90.00, 100.00, 90.00

Refinement procedure

Resolution	10.000 - 1.800
R-factor	0.187
Rwork	0.187
R-free	0.21300
Structure solution method	MULTIPLE ISOMORPHOUS REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	22.830 *
Data reduction software	WEIS
Data scaling software	CCP4
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]		1.880
High resolution limit [Å]	1.800	1.790
Rmerge	0.059	0.297
Total number of observations	180543 *
Number of reflections	39895 *
<I/σ(I)>		2.6
Completeness [%]	91.3	74.4
Redundancy	3.3	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	8.5	25 *	pH 8.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir	PEG4000	30 (%(w/v))
3	1	reservoir	sodium acetate	0.2 (M)
4	1	reservoir	FMN	0.1 (mM)
5	1	reservoir	Tris-HCl	100 (mM)