1VFG
Crystal structure of tRNA nucleotidyltransferase complexed with a primer tRNA and an incoming ATP analog
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2003-12-09 |
Detector | RIGAKU RAXIS V |
Wavelength(s) | 1.000, 0.9793, 0.9795, 0.9843, 0.9743 |
Spacegroup name | P 1 2 1 |
Unit cell lengths | 109.476, 125.900, 58.920 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.000 - 2.800 |
Rwork | 0.230 |
R-free | 0.28650 |
Structure solution method | MAD |
RMSD bond length | 0.012 |
RMSD bond angle | 1.520 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MLPHARE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.870 |
High resolution limit [Å] | 2.800 | 2.800 |
Number of reflections | 39382 | |
<I/σ(I)> | 22.4 | 4.3 |
Completeness [%] | 99.2 | 95.7 |
Redundancy | 6.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | MPD, magnesium acetate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | MPD | ||
2 | 1 | 1 | magnesium acetate | ||
3 | 1 | 2 | MPD | ||
4 | 1 | 2 | magnesium acetate |