1VF8
The Crystal Structure of Ym1 at 1.31 A Resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL17B2 |
Synchrotron site | NSRRC |
Beamline | BL17B2 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2003-04-05 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 50.611, 60.123, 60.106 |
Unit cell angles | 90.00, 94.67, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.310 |
R-factor | 0.175 |
Rwork | 0.175 |
R-free | 0.19700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1e9l |
RMSD bond length | 0.012 |
RMSD bond angle | 1.990 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.360 |
High resolution limit [Å] | 1.310 | 1.310 |
Rmerge | 0.068 | 0.253 |
Number of reflections | 79157 | |
<I/σ(I)> | 13.4 | 3.2 |
Completeness [%] | 91.7 | 93.7 |
Redundancy | 2.3 | 2.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICRODIALYSIS | 7 | 277 | distilled water, pH 7.0, MICRODIALYSIS, temperature 277K |