1VF6
2.1 Angstrom crystal structure of the PALS-1-L27N and PATJ L27 heterodimer complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-03-17 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0722 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 111.060, 111.060, 193.690 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.100 |
| R-factor | 0.242 |
| Rwork | 0.242 |
| R-free | 0.26200 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.918 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.200 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 25593 | |
| Completeness [%] | 94.3 | 99.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293.5 | ammonium sulfate, glycerol, CHAPS, sodium citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293.5K |
