1VF2
cGSTA1-1 in complex with S-hexyl-glutathione
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-01-17 |
| Detector | RIGAKU RAXIS IIC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 101.316, 115.346, 95.145 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.280 - 2.150 |
| Rwork | 0.204 |
| R-free | 0.23800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1guh |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.100 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.280 | 2.250 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.078 | 0.406 |
| Number of reflections | 30228 | |
| <I/σ(I)> | 9.2 | 2.23 |
| Completeness [%] | 98.4 | 89.1 |
| Redundancy | 7.46 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 298 | sodium cacodylate, magnesium acetate, zinc acetate, PEG8000, pH 6.5, VAPOR DIFFUSION, temperature 298K |
