1VE8
X-Ray analyses of oligonucleotides containing 5-formylcytosine, suggesting a structural reason for codon-anticodon recognition of mitochondrial tRNA-Met; Part 1, d(CGCGAATT(f5C)GCG)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-05-25 |
Detector | ADSC QUANTUM 9R |
Wavelength(s) | 0.9 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 25.100, 41.600, 65.500 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 9.000 - 1.650 |
Rwork | 0.221 |
R-free | 0.27900 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 0.900 |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.100 | 1.700 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.060 | 0.297 |
Number of reflections | 9005 | |
<I/σ(I)> | 2.7 | |
Completeness [%] | 99.5 | 99.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 2-methyl-2,4-pentanediol, spermine tetrahydrochloride, barium chloride, sodium chloride, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | 2-methyl-2,4-pentanediol | ||
2 | 1 | 1 | spermine tetrahydrochloride | ||
3 | 1 | 1 | BaCl | ||
4 | 1 | 1 | NaCl | ||
5 | 1 | 1 | H2O | ||
6 | 1 | 2 | BaCl | ||
7 | 1 | 2 | NaCl | ||
8 | 1 | 2 | H2O |