1VE1

Crystal Structure of T.th. HB8 O-acetylserine sulfhydrylase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44B2
Synchrotron site	SPring-8
Beamline	BL44B2
Temperature [K]	100
Detector technology	CCD
Collection date	2001-10-15
Detector	MARRESEARCH
Wavelength(s)	1.0
Spacegroup name	P 31 2 1
Unit cell lengths	104.470, 104.470, 79.670
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	12.700 - 1.450
Rwork	0.220
R-free	0.24900
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.260
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.500
High resolution limit [Å]	1.450	1.450
Number of reflections	86533
Completeness [%]	97.4	98

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	Ammonium Sulfate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K