1VE0

Crystal structure of uncharacterized protein ST2072 from Sulfolobus tokodaii

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44B2
Synchrotron site	SPring-8
Beamline	BL44B2
Temperature [K]	100
Detector technology	CCD
Collection date	2003-11-23
Detector	ADSC
Wavelength(s)	0.9789, 0.9794, 0.9000
Spacegroup name	P 21 3
Unit cell lengths	71.720, 71.720, 71.720
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.000
Rwork	0.181
R-free	0.22200
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.417
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.070
High resolution limit [Å]	2.000	2.000
Number of reflections	8590
<I/σ(I)>	10.6
Completeness [%]	100.0	100
Redundancy	21.5	21.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.2	293	0.1M Tris-HCl pH8.2, 20% PEG4000, 0.2M LiSO4, VAPOR DIFFUSION, HANGING DROP, temperature 293K