1VDZ

Crystal structure of A-type ATPase catalytic subunit A from Pyrococcus horikoshii OT3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2002-05-17
Detector	MARRESEARCH
Wavelength(s)	0.9000
Spacegroup name	P 43 21 2
Unit cell lengths	127.977, 127.977, 104.732
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.550
R-factor	0.242
Rwork	0.239
R-free	0.27100
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.300
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.640
High resolution limit [Å]	2.550	2.550
Number of reflections	28944
<I/σ(I)>	3.9	2
Completeness [%]	99.9	99.9
Redundancy	10.8	11.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	293	MPD, acetate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K
1	VAPOR DIFFUSION, HANGING DROP	4.5	293	MPD, acetate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K