1VCR

An icosahedral assembly of light-harvesting chlorophyll a/b protein complex from pea thylakoid membranes

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2000-05-22
Detector	MARRESEARCH
Wavelength(s)	1.000
Spacegroup name	F 2 3
Unit cell lengths	360.646, 360.646, 360.646
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 9.500
Rwork	0.379
R-free	0.35300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Personal communication with Prof. W. Kuhlbrandt. Nature 367 614-621 (1994)
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	500.000	10.100
High resolution limit [Å]	9.500	9.500
Rmerge		0.499
Number of reflections	2532
<I/σ(I)>	3	1.5
Completeness [%]	99.9	99.9
Redundancy	8	8.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	288	Potassium chloride, PEG4000, Xylitol, HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 288K
1	VAPOR DIFFUSION, HANGING DROP	7	288	Potassium chloride, PEG4000, Xylitol, HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 288K
1	VAPOR DIFFUSION, HANGING DROP	7	288	Potassium chloride, PEG4000, Xylitol, HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 288K