1VCK

Crystal structure of ferredoxin component of carbazole 1,9a-dioxygenase of Pseudomonas resinovorans strain CA10

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Temperature [K]	100
Detector technology	CCD
Collection date	2001-04-07
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0
Spacegroup name	P 41 3 2
Unit cell lengths	98.281, 98.281, 98.281
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.630 - 1.900
R-factor	0.202
Rwork	0.202
R-free	0.23600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	1.300
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	MOLREP
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.630	2.020
High resolution limit [Å]	1.900	1.900
Number of reflections	13347
Completeness [%]	99.9	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	278	17-19% PEG2000 MME, 0.2M ammonium sulfate, 0.1M sodium acetate, 0.02M magnesium chloride, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 278K