1VCH
Crystal Structure of a Phosphoribosyltransferase-related protein from Thermus thermophilus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2003-01-04 |
Detector | RIGAKU RAXIS |
Wavelength(s) | 0.976, 0.97935, 0.982, 0.97904, 0.973 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 167.419, 61.405, 102.389 |
Unit cell angles | 90.00, 93.97, 90.00 |
Refinement procedure
Resolution | 44.980 - 1.940 |
R-factor | 0.232 |
Rwork | 0.232 |
R-free | 0.25900 |
Structure solution method | MOLECULAR REPLACEMENT, MAD |
Starting model (for MR) | Partial structure from MAD data set |
RMSD bond length | 0.006 |
RMSD bond angle | 1.400 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.053 | 0.501 |
Number of reflections | 80673 | |
<I/σ(I)> | 19.8 | 2.1 |
Completeness [%] | 98.7 | 98.2 |
Redundancy | 3.6 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 291 | MES, PEG 10K, CaAc, MeOH, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 291 | MES, PEG 10K, CaAc, MeOH, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 291K |