1VCG
Crystal Structure of IPP isomerase at P43212
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL44B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-12-04 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9803 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 112.953, 112.953, 261.283 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.980 - 3.020 |
| R-factor | 0.24 |
| Rwork | 0.240 |
| R-free | 0.26200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.800 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.120 |
| High resolution limit [Å] | 3.010 | 3.010 |
| Number of reflections | 32106 | |
| <I/σ(I)> | 24.1 | 3.1 |
| Completeness [%] | 93.4 | 65.9 |
| Redundancy | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | sodium acetate, sodium chloride, MPD, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
