1VCD
Crystal Structure of a T.thermophilus HB8 Ap6A hydrolase Ndx1
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL45XU |
| Synchrotron site | SPring-8 |
| Beamline | BL45XU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-05-22 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.0, 0.9744, 0.9792, 0.9798, 0.9819 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 49.559, 52.324, 114.009 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.760 - 1.700 |
| R-factor | 0.214 |
| Rwork | 0.214 |
| R-free | 0.23700 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.048 | 0.280 |
| Number of reflections | 33444 | |
| <I/σ(I)> | 33.1 | 7.8 |
| Completeness [%] | 99.9 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 293 | 0.1 MHEPES, 2%(w/v) PEG 400, 1.4M ammonium sulfate, 20% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 293K, pH 7.6 |
