1VC1
Crystal structure of the TM1442 protein from Thermotoga maritima, a homolog of the Bacillus subtilis general stress response anti-anti-sigma factor RsbV
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-18B |
Synchrotron site | Photon Factory |
Beamline | BL-18B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1999-11-27 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 31.544, 116.831, 31.392 |
Unit cell angles | 90.00, 119.84, 90.00 |
Refinement procedure
Resolution | 19.920 - 2.000 |
R-factor | 0.205 |
Rwork | 0.205 |
R-free | 0.21100 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.014 |
RMSD bond angle | 1.700 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.130 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 12842 | |
Completeness [%] | 96.4 | 94.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | 100mM sodium acetate, 100mM magnesium chloride, 18%(v/v) polyethylene glycol 400, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |