1VBX
Crystal Structure of the Hepatitis Delta Virus Gemonic Ribozyme Precursor, with C75U mutaion, in EDTA solution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2003-03-30 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0781 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 108.581, 108.581, 189.996 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.230 - 2.700 |
| R-factor | 0.252 |
| Rwork | 0.252 |
| R-free | 0.28500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.230 | 2.870 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 11760 | |
| Completeness [%] | 97.1 | 93.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | NaCl, EDTA, MPD, Sodium Cacodylate, Spermine-HCl, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | NaCl | ||
| 2 | 1 | 1 | EDTA | ||
| 3 | 1 | 1 | MPD | ||
| 4 | 1 | 1 | Sodium Cacodylate | ||
| 5 | 1 | 1 | Spermine-HCl | ||
| 6 | 1 | 2 | NaCl | ||
| 7 | 1 | 2 | EDTA | ||
| 8 | 1 | 2 | MPD | ||
| 9 | 1 | 2 | Sodium Cacodylate |
