1VBO
Crystal structure of artocarpin-mannotriose complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 278 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-06-10 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 138.217, 72.296, 59.373 |
| Unit cell angles | 90.00, 94.48, 90.00 |
Refinement procedure
| Resolution | 19.850 - 2.350 |
| R-factor | 0.209 |
| Rwork | 0.209 |
| R-free | 0.22600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1j4u |
| RMSD bond length | 0.018 |
| RMSD bond angle | 3.400 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.430 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.104 | 0.498 |
| Number of reflections | 48504 | |
| <I/σ(I)> | 8.9 | |
| Completeness [%] | 99.2 | 99.3 |
| Redundancy | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 300 | PEG4000, PEG1450, Phosphate buffer, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
