1VBM
Crystal structure of the Escherichia coli tyrosyl-tRNA synthetase complexed with Tyr-AMS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-01-31 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9843 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 83.189, 83.189, 182.424 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.540 - 2.700 |
| R-factor | 0.246 |
| Rwork | 0.246 |
| R-free | 0.28400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1UDF |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.800 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.061 | 0.189 |
| Number of reflections | 23041 | |
| <I/σ(I)> | 23.4 | 3.6 |
| Completeness [%] | 88.3 | 81.2 |
| Redundancy | 6.2 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | ammonium sulphate, PEG 400, sodium chloride, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
