1VBK
Crystal structure of PH1313 from Pyrococcus horikoshii Ot3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2003-11-30 |
Detector | RIGAKU RAXIS V |
Wavelength(s) | 1.00000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 71.743, 71.164, 141.774 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.470 - 1.900 |
R-factor | 0.203 |
Rwork | 0.199 |
R-free | 0.22200 |
Structure solution method | SAD |
RMSD bond length | 0.005 |
RMSD bond angle | 1.200 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.077 | 0.538 |
Number of reflections | 57832 | |
<I/σ(I)> | 9 | 3.81 |
Completeness [%] | 99.9 | 100 |
Redundancy | 5.7 | 5.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 5.4 | 291 | MRD, pH 5.4, MICROBATCH, temperature 291K |
1 | MICROBATCH | 5.4 | 291 | MRD, pH 5.4, MICROBATCH, temperature 291K |