1VBJ
The crystal structure of prostaglandin F synthase from Trypanosoma brucei
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL12B2 |
Synchrotron site | SPring-8 |
Beamline | BL12B2 |
Temperature [K] | 100 |
Wavelength(s) | 0.68811 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 113.212, 113.212, 136.269 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.620 - 2.100 |
R-factor | 0.208 |
Rwork | 0.208 |
R-free | 0.26000 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.200 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.071 | 0.248 |
Number of reflections | 47485 | |
<I/σ(I)> | 7.1 | |
Completeness [%] | 91.1 | 88.4 |
Redundancy | 8.9 | 8.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | sodium citrate, pH 6.5, VAPOR DIFFUSION, HANGING DROP |