1VBI
Crystal structure of type 2 malate/lactate dehydrogenase from thermus thermophilus HB8
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-02-03 |
| Detector | RIGAKU RAXIS V |
| Wavelength(s) | 0.97900, 0.97931, 1.0000 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 78.320, 78.320, 253.213 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 1.800 |
| R-factor | 0.188 |
| Rwork | 0.187 |
| R-free | 0.21000 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.061 | 0.130 |
| Number of reflections | 43745 | |
| <I/σ(I)> | 16.3 | 11.53 |
| Completeness [%] | 99.9 | 99.6 |
| Redundancy | 13 | 8.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 4.6 | 295 | 1.58M ammonium sulfate, 0.1M sodium acetate, 1mM NAD, pH 4.6, microbatch, temperature 295.0K |
