1VBH
Pyruvate Phosphate Dikinase with bound Mg-PEP from Maize
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL45PX |
Synchrotron site | SPring-8 |
Beamline | BL45PX |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2000-12-12 |
Detector | RIGAKU RAXIS V |
Wavelength(s) | 1.02 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 109.339, 100.510, 108.155 |
Unit cell angles | 90.00, 98.48, 90.00 |
Refinement procedure
Resolution | 45.630 - 2.300 |
R-factor | 0.238 |
Rwork | 0.230 |
R-free | 0.25900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1vbg |
RMSD bond length | 0.008 |
RMSD bond angle | 1.200 |
Data scaling software | CrystalClear ((MSC/RIGAKU)) |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.630 | 2.440 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.075 | 0.255 |
Number of reflections | 51391 | |
<I/σ(I)> | 7.5 | 2.5 |
Completeness [%] | 99.9 | 99 |
Redundancy | 4.04 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | PEG8000, magnesium sulfate, glycerol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |