1VBF

Crystal structure of protein L-isoaspartate O-methyltransferase homologue from Sulfolobus tokodaii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2002-07-04
Detector	MARRESEARCH
Wavelength(s)	0.9793
Spacegroup name	F 2 3
Unit cell lengths	277.803, 277.803, 277.803
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 2.800
Rwork	0.205
R-free	0.25500
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.656
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.037	2.950
High resolution limit [Å]	2.800	2.800
Number of reflections	43276
<I/σ(I)>	7.9	2.2
Completeness [%]	100.0	100
Redundancy	22.9	22.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	293	lithium sulfate, ammonium sulfate, citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K