1VB6
Crystal Structure of the heme PAS sensor domain of Ec DOS (oxygen-bound form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-03-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.71 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.894, 69.337, 81.876 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.410 - 1.560 |
| R-factor | 0.214 |
| Rwork | 0.214 |
| R-free | 0.23200 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1v9y |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS (1.1) |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.630 |
| High resolution limit [Å] | 1.560 | 1.560 |
| Number of reflections | 39232 | |
| Completeness [%] | 98.0 | 96.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | PEG8000, MgCl2, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
