1VB4
T=1 capsid structure of Sesbania mosaic virus coat protein deletion mutant CP-N(delta)36
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-07-29 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 187.302, 193.308, 268.368 |
| Unit cell angles | 90.00, 92.28, 90.00 |
Refinement procedure
| Resolution | 15.000 - 3.300 |
| R-factor | 0.255 |
| Rwork | 0.255 |
| R-free | 0.25900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | GLRF |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 3.500 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.156 | 0.226 |
| Number of reflections | 205217 | |
| Completeness [%] | 71.2 | 73.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 290 | PEG 3350, magnesium chloride, isopropanol , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
