1VAX

Crystal Structure of Uricase from Arthrobacter globiformis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2003-12-17
Detector	ADSC QUANTUM 210
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	85.430, 122.540, 284.460
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 1.990
R-factor	0.193
Rwork	0.193
R-free	0.22300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1UOX
RMSD bond length	0.005
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.220	2.060
High resolution limit [Å]	1.990	1.990
Rmerge	0.060	0.177
Number of reflections	363070
<I/σ(I)>	12.3	4.7
Completeness [%]	91.8	87.1
Redundancy	3.47	3.34

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	9	277	PEG 8000, Lithium sulphate, pH 9.0, VAPOR DIFFUSION, temperature 277K