1VAV
Crystal structure of alginate lyase PA1167 from Pseudomonas aeruginosa at 2.0 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-03-14 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.359, 70.219, 67.299 |
| Unit cell angles | 90.00, 94.77, 90.00 |
Refinement procedure
| Resolution | 37.780 - 2.000 |
| R-factor | 0.187 |
| Rwork | 0.187 |
| R-free | 0.24000 |
| Structure solution method | MIR |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASES |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.106 | 0.516 |
| Number of reflections | 30777 | |
| Completeness [%] | 88.9 | 43.8 |
| Redundancy | 4 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | NaCl, Potassium sodium phosphate, MES-sodium phosphate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
