1VAT
Iodine derivative of hen egg-white lysozyme
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-04-26 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.5400, 0.3746, 0.3734 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 78.350, 78.350, 37.540 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.600 |
| Rwork | 0.205 |
| R-free | 0.23200 |
| Structure solution method | MAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 15776 | |
| <I/σ(I)> | 32.2 | 5.5 |
| Completeness [%] | 99.5 | 99.9 |
| Redundancy | 12.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 50mg/ml HEWL, 20%(w/v) PEG4000, 100mM sodium citrate (pH 5.5), 100mM sodium iodide, and 20%(v/v) glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
