1VAL

CONCANAVALIN A COMPLEX WITH 4'-NITROPHENYL-ALPHA-D-GLUCOPYRANOSIDE

Experimental procedure

Detector technology	IMAGE PLATE
Collection date	1994-10-20
Detector	MARRESEARCH
Spacegroup name	P 21 21 2
Unit cell lengths	134.660, 155.670, 71.420
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 3.000
R-factor	0.186
Rwork	0.186
R-free	0.27400
RMSD bond length	0.006
RMSD bond angle	26.800 *
Data reduction software	XDS
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.000
High resolution limit [Å]	3.000	3.000 *
Rmerge	0.143	0.439 *
Total number of observations	116891 *
Number of reflections	30575
Completeness [%]	99.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8.5 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	Tris	10 (mM)
2	1	reservoir	ammonium sulfate	1 (M)
3	1	drop	ConA	70 (mg/ml)
4	1	drop	saccharide		10 * molar excess