1VAK
T=1 capsid structure of Sesbania mosaic virus coat protein deletion mutant CP-N(delta)65
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-07-10 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 188.239, 193.954, 269.054 |
| Unit cell angles | 90.00, 92.42, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.050 |
| R-factor | 0.211 |
| Rwork | 0.211 |
| R-free | 0.21400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | GLRF |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.160 |
| High resolution limit [Å] | 3.050 | 3.050 |
| Rmerge | 0.080 | 0.122 |
| Number of reflections | 348643 | |
| Completeness [%] | 53.0 | 95.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | PEG 3350, magnesium chloride, isopropanol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
