1VAJ
Crystal Structure of Uncharacterized Protein PH0010 From Pyrococcus horikoshii
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-12-21 |
| Wavelength(s) | 0.9742, 0.9785, 0.9600 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 60.672, 67.223, 58.728 |
| Unit cell angles | 90.00, 104.27, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.820 |
| Rwork | 0.202 |
| R-free | 0.23200 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.410 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.630 | 1.890 |
| High resolution limit [Å] | 1.820 | 1.820 |
| Rmerge | 0.071 | 0.336 |
| Number of reflections | 19449 | |
| <I/σ(I)> | 10.6 | |
| Completeness [%] | 95.7 | 77.2 |
| Redundancy | 5.4 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.9 | 293 | PEG 8000, magnesium chloride, pH 7.9, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
