1VA5
Antigen 85C with octylthioglucoside in active site
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2000-08-04 |
Detector | MACSCIENCE |
Wavelength(s) | 1.5418 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 144.818, 68.117, 82.281 |
Unit cell angles | 90.00, 119.60, 90.00 |
Refinement procedure
Resolution | 38.720 - 2.020 |
R-factor | 0.187 |
Rwork | 0.187 |
R-free | 0.21100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1dzq |
RMSD bond length | 0.006 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.720 | 2.120 |
High resolution limit [Å] | 2.020 | 2.020 |
Number of reflections | 44080 | |
<I/σ(I)> | 17.5 | |
Completeness [%] | 95.2 | 83.9 |
Redundancy | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | octylthioglucoside, Sodium Acetate, Imidazole, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |