1VA5
Antigen 85C with octylthioglucoside in active site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-08-04 |
| Detector | MACSCIENCE |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 144.818, 68.117, 82.281 |
| Unit cell angles | 90.00, 119.60, 90.00 |
Refinement procedure
| Resolution | 38.720 - 2.020 |
| R-factor | 0.187 |
| Rwork | 0.187 |
| R-free | 0.21100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dzq |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.720 | 2.120 |
| High resolution limit [Å] | 2.020 | 2.020 |
| Number of reflections | 44080 | |
| <I/σ(I)> | 17.5 | |
| Completeness [%] | 95.2 | 83.9 |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | octylthioglucoside, Sodium Acetate, Imidazole, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
