1VA0
Crystal Structure of the Native Form of Uroporphyrin III C-methyl transferase from Thermus thermophilus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-12-03 |
| Detector | RIGAKU RAXIS VII |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 60.500, 63.568, 131.902 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.780 - 1.970 |
| R-factor | 0.199 |
| Rwork | 0.199 |
| R-free | 0.22700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1cbf |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.600 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.780 | 2.040 |
| High resolution limit [Å] | 1.970 | 1.970 |
| Rmerge | 0.039 | 0.494 |
| Number of reflections | 36786 | |
| <I/σ(I)> | 18.8 | 1.65 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 3.6 | 3.53 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 291 | Citrate, PEG 20000, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
