1V9Y
Crystal Structure of the heme PAS sensor domain of Ec DOS (ferric form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-03-16 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.71 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.740, 69.780, 82.540 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.320 |
| R-factor | 0.16 |
| Rwork | 0.160 |
| R-free | 0.21930 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 0.030 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.390 |
| High resolution limit [Å] | 1.320 | 1.320 |
| Number of reflections | 60488 | |
| <I/σ(I)> | 23.9 | 1.9 |
| Completeness [%] | 98.6 | 100 |
| Redundancy | 5.8 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 295 | ammonium sulfate, MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
