1V9T
Structure of E. coli cyclophilin B K163T mutant bound to succinyl-ALA-PRO-ALA-P-nitroanilide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-6A |
Synchrotron site | Photon Factory |
Beamline | BL-6A |
Temperature [K] | 293 |
Detector technology | DIFFRACTOMETER |
Collection date | 1999-01-01 |
Detector | WEISSENBERG |
Wavelength(s) | 1.0 |
Spacegroup name | P 32 |
Unit cell lengths | 79.280, 79.280, 56.630 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 6.000 - 1.700 |
Rwork | 0.184 |
R-free | 0.22100 |
Structure solution method | MIR |
Starting model (for MR) | 1lop |
RMSD bond length | 0.006 |
RMSD bond angle | 1.230 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 80.000 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.090 | 0.238 |
Number of reflections | 40355 | |
<I/σ(I)> | 16.7 | |
Completeness [%] | 92.0 | 73.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | ammonium sulfate, methanol, sodium azide, Tris-HCl, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |