1V9N

Structure of Malate Dehydrogenase from Pyrococcus horikoshii OT3

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B1
Synchrotron site	SPring-8
Beamline	BL26B1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2003-11-01
Detector	RIGAKU RAXIS
Wavelength(s)	0.985, 0.97914, 0.97944
Spacegroup name	P 31 2 1
Unit cell lengths	74.190, 74.190, 127.280
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.680 - 2.100
R-factor	0.208
Rwork	0.208
R-free	0.24300
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	1.300
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.230
High resolution limit [Å]	2.100	2.100
Rmerge	0.073
Number of reflections	24293
Completeness [%]	99.7	100
Redundancy	5.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	5.1	295	PEG, HEPES, LiCl2, pH 5.1, MICROBATCH, temperature 295K